NTU BIME

Lin-Chi  Chen
Full-Time
Name Lin-Chi Chen
Job Title Professor and Chair
Deputy Vice President for Student Affairs
Division Director, Student Activity Division
Email chenlinchi@ntu.edu.tw
Office Tel No. +886-2-3366-5339; 3366-5335
Research Expertise DNA and Aptamer Electrochemical sensors and biosensors Biochips nano materials
Teaching Field Unit Operation in Bio-industry Biomolecular Sensing Devive Bioprocess Engineering Introduction to biochemical reaction engineering Introduction to Experimental Molecular Biology Methods biochemistry Physical Chemistry Biology for Engineers
Laboratory Website http://chenlinchi.wixsite.com/ntubmdlab
Recent Publications in Electrochemical Sensing Fields
  1. H.-Y. Liao, T.-C. Liao, W.-H. Chen, C.-H. Chang, and L.-C. Chen*, “Molybdate hexacyano- ferrate (MoOHCF) thin film: A brownish red Prussian blue analog for electrochromic window application,” Sol. Energ. Mat. Sol. Cells.145, 8-15 (2016).
  2. T.-C. Liao, W.-H. Chen, H.-Y. Liao, and L.-C. Chen*, “Multicolor electrochromic thin films and devices based on the Prussian blue family nanoparticles,” Sol. Energ. Mat. Sol. Cells.145, 26-34 (2016).
  3. C.-C. Chen and L.-C. Chen*, “Synthesis and characterization of Pd-Ni core-shell nano-catalysts for alkaline glucose electrooxidation,” RSC Adv5 , 53333-53339 (2015).
  4. C.-C. Chen, C.-L. Lin, and L.-C. Chen*, “A binary palladium-bismuth nanocatalyst with high activity and stability for alkaline glucose electrooxidation,” J. Power Sources287, 323-333 (2015).
  5. C.-C. Chen, C.-L. Lin, and L.-C. Chen*, “Functionalized carbon nanomaterial supported palladium nano-catalysts for electrocatalytic glucose oxidation reaction,” Electrochim 152, 408-416 (2015).
  6. J.-Y. Wang, Chou, T.-C. Chou, L.-C. Chen*, K.-C. Ho*, “Using poly(3-aminophenylboronic acid) thin film with binding-induced ion flux blocking for amperometric detection of hemoglobin A1c,” Biosens. Bioelectron63, 317-324 (2015).
Recent Publications in Aptamer and DNA Fields

 
  1. Weng, J.-H., and L.-C. Chen*, “Confocal epifluorescence sensor with an arc-shaped aperture for slide-based PCR quantification,” Biosensors and Bioelectronics 100, 71-78 (2018). 
  2. Yang, D.-K., Chou, C.-F., Chen, L.-C., "Selection of aptamers for AMACR detection from DNA libraries with different primers", RSC Adv. 8, Issue 34, 19067-19074 (2018).
  3. Jolly, P.,  Miodek, A., Yang, D.-K., Chen, L.-C., Lloyd, M.D., Estrela, P., “Electro-Engineered Polymeric Films for the Development of Sensitive Aptasensors for Prostate Cancer Marker Detection”, ACS Sensors 1, Issue 11, 1308-1314 (2016).
  4. Xu, C.-H., Shen, P.-Y., Chiu, Y.-F., Yeh, P.-W., Chen, C.-C., Chen, L.-C., Hsu, C.-C., Cheng, I.-C.,  Chen, J.-Z., "Atmospheric pressure plasma jet processed nanoporous Fe2O3/CNT composites for supercapacitor application", Journal of Alloys and Compounds 676, 469-473 (2016).
  5. Y.-H. Lao, C.-W. Chi. S. M. Friedrich, K. Peck, T.-H. Wang, K. W. Leong*, and L.-C. Chen*, “Signal-on protein detection via dye translocation between aptamer and quantum dot,” ACS Appl. Mater. Interfaces 8, 12048–12055 (2016).
  6. S.-J. Wang, Y.-T. Hou, and L.-C. Chen*, “A selective decoy-doxorubicin complex for targeted co-delivery, STAT3 probing and synergistic anti-cancer effect,” Chem. Commun.51, 13309- 13312 (2015).
  7. D.-K. Yang, C.-J. Kuo, and L.-C. Chen*, “Synthetic multivalent DNAzymes for enhanced hydrogen peroxide catalysis and sensitive colorimetric glucose detection,” Anal. Chim. Acta. 856, 96-102 (2015).
  8. D.-K. Yang, L.-C. Chen*, M.-Y. Lee, C.-H. Hsu, and C.-S. Chen, “Selection of aptamers for fluorescent detection of alpha-methylacyl-CoA racemase by single-bead SELEX,” Biosens. Bioelectron. 62, 106-112 (2014).
  9. Y.-H. Lao, H.-Y. Chiang, D.-K. Yang, K. Peck, and L.-C. Chen*, “Selection of aptamers targeting the sialic acid receptor of hemagglutinin by epitope-specific SELEX,” Chem. Comm50, 8719-8722 (2014).
  10. S.-J. Wang, C.-S. Chen, and L.-C. Chen*, “Prussian blue nanoparticles as nanocargoes for delivering DNA drugs to cancer cells,” Sci. Technol. Adv. Mat. 14, 044405 (2013).
Year Paper Title
2018 Chih-Yu Lai, Lin-Chi Chen*, EIS Detection of MUC1 with Two Symmetric Aptamer/Au Electrodes, 22nd Topical meeting of the international society of electrochemistry, 日本東京早稻田大學
2018 Li-Chen Ho, Lin-Chi Chen*, Effects of Anion Doping on the Surface Hydrophobicity and Pseudo-capacitance of a Poly(3,4-ethylenedioxythiophene) Solid Contact, 22nd Topical meeting of the international society of electrochemistry, 日本東京早稻田大學
2017 Ching-Jung Yen, Lin-Chi Chen*, Probing the Water Layer Effect of a Solid-Contact Ion-Selective Electrode by Fluorescent Confocal Microscopy, 第19屆歐洲分析化學研討會 2017
2017 Pei-Wei Lee, Lin-Chi Chen*, Simultaneous Targeting STAT3 and NF-κB in Cancer Cell Lines with Decoy Oligonucleotide, 第254屆ACS國際研討會
2017 Jui-Hong Weng, Lin-Chi Chen*, 1D Bead-Array SELEX for STAT3-Targeted Aptamer Selection, 第254屆ACS國際研討會
2017 Wan-Ju Chen, Lin-Chi Chen*, Synergistic effect of STAT3 and NF-B decoy oligonucleotides on prostate cancer cell inhibition, 第254屆ACS國際研討會
2017 Yu-Fu Chen, Lin-Chi Chen, Tuning the Hydrophobicity of Polyaniline Solid Contact for Improving Ion Sensing Performance, 第19屆歐洲分析化學研討會
2017 Jou-Hsuan Chu, Lin-Chi Chen*, Functionalized conducting polymer electrodes for aptasensor fabrication, 台灣化學工程學會64周年年會暨科技部化學工程學門成果發表會
2017 Chih-Yu Lai, Lin-Chi Chen, The Study of an Impedimetric Microfluidic Chip Design for Mucin1 Aptasensing, 台灣化學工程學會64周年年會暨科技部化學工程學門成果發表會
2017 Yi-Min Wu, Chih-Hao Chen, Ching-Jung Yen, and Lin-Chi Chen*, Study of solid-state reference electrodes with antifouling property for multi-ion detection in protein-rich samples, 台灣化學工程學會64周年年會暨科技部化學工程學門成果發表會
2017 Jui-Hong Weng, Chih-Yu Lai, Lin-Chi Chen*, Shuttling microfluidic platform for rapid electrochemical analysis, 2017綠色電化學科技學術研討會暨2017年台灣電化學學會
 
Year Project name Paticipant Title Year associated agency 備註
2016 Mouse-on-a-Chip Establishment and Frontier Biotech Applications - Interactome Analysis, Pesticide Toxicology Research and DNA Anti-cancer Drug Development Lin-Chi Chen PI 2016 ~  MOST  
2018 Advanced developing of electrochemical ion-sensing chip - principle investigation, smart sensing and promising application Lin-Chi Chen PI 2018~  MOST
2017 Construction of the selection platform of aptamers and looped oligonucleotide by application of nanosized optofluidics and bioinfomation Lin-Chi Chen PI 2017 ~
MOST
Period

DNA and Aptamer

  1. CHEN, LIN CHI; LIAO, HUNG YUN; CHI, CHUN WEI; LAO, YEH HSING; HUANG, CHEN KANG; CHUANG, CHI YU; CHANG, CHENG PING; HUNG, PO CHEN; HSIEH, SHU JUNG; CHEN, CHIOU JONG"聚合脢連鎖反應儀光學偵測模組" , 中華民國專利號碼M393517, 2010年
  2. HUANG, CHEN KANG; LEE, CHIH WEI; CHEN, LIN CHI; CHUANG, CHI YU; CHANG, CHENG PING; HUNG, PO CHEN; HSIEH, SHU JUNG; CHEN, CHIOU JONG"qPCR加熱降溫模組" , 中華民國專利號碼M393516, 2010年
  3. CHEN, LIN CHI; KAO, CHUN FENG; CHI, CHUN WEI"自動純化核酸裝置" , 中華民國專利公報, 證書號數:M356741, Int. Cl. : C12N15/10(2006.01) , 公告日: 中華民國98(2009)年5月11日, 2009年05月

Electrochemistry

  1. CHEN, LIN CHI; CHENG, TZONG JIH; FANG, WEI; CHEN, JIAN ZHANG; YEN, CHING JUNG; PAN, CHING YU; WU, PO LIN; YANG, CHANG HAN; HUANG, SHENG FENG; SHIH, WEI LI; WU, YI MIN; LU, CHI HAN "A MULTI-ION SENSING ELECTRODE ARRAY CHIP AND SENSING DEVICE THEREOF", 中華民國專利號碼M565302, 2018年
  2. ​陳林祈;余宗穆 ;顏妙儒, "用於生物燃料電池之生物陽極電極、製備方法及其應用" , 中華民國申請號201128843, 2011年
  3. ​王安邦; 王希俊; 李君浩; 蘇國棟; 陳林祈; 湯競恆; 林剛誼, "Educational Kit for Display Device and Educational Method Utilizing the Same" , 美國專利20100110325, 2010年

  4. 王安邦;王希俊;李君浩;蘇國棟;陳林祈;湯競恒;林岡誼, "顯示裝置之教學組件以及應用教學組件之教學方法" , 中華民國申請號201019286, 2010年

  5. 陳林祈、洪祥富, "可調整光穿透率之節能片材裝置" , 中華民國專利公報, 證書號數:M351910, Int. Cl. : E04C2/26 (2006.01) , 公告日: 中華民國98(2009)年3月1日, 2009年03月

  6. 陳林祈;洪祥富;柯旻利;王安邦;陳國慶, "電致色變實驗教學套組" , 中華民國申請號200944914, 2009年

Biochip

  1. CHOU, CHENG CHUNG; CHEN, LIN CHI; WU, JUI YU; LAO, YEH HSING; KAO, CHUN FENG; CHI, CHUN WEI"可溫控之微型生物分子反應晶片" , 中華民國專利號碼M373489, 2012年05月
DNA and Aptamer
Aptamers are ligands made with artificial systematic evolution. The artificial selection of the nucleic acid gene bank with high diverisity is done by the stp of selection-elimination-ampflication to pick up the short DNA part being able to bind with the sensing target. Our eam has developed unique technique for selection of aptamers, enable people to find the aptamer with good affinity in a short time, and applied these to the biosensor, disease of detection, and medical treatment.

Electrochemical sensing
Our team  endeavor to develope a multiplex ion sensing chip and method with high accuracy and low-cost. There are multi-ion sensing chip, fixture  mechanism(for the chip), and APP in our integrated system. The multi-ion sensing chip includes solid-state reference electrodes and ion sensing electrodes, which can be made with screen printing or semi-conductor processing. Our team is working on the miniaturization of the multi-ion sensing chip. The APP can easily perform calibration and measurement of multi-ions in a short time with user-friendly user interface. The availability of oue system has been tested by practical using in plant factory.

Biochip
The advantages of biochip are reduction of fluid volume and high flux, which contributes to the effeciency of biological analysis. Our team has developed a biochip based on a new type of cell to the proof of DNA drugs and the analysis of cellular metabolism. This chip is user friendly; operation performed by researchers in other field can be easy. As a result, there is potential of our chip to be integrated with other techniques. Our team has been cultivating the rat liver cell and breast cancer cell in our chip, and analyzing the efficacy and toxicity of some drugs.
University Country Dept Degree Year
National Taiwan University Taiwan Chemical Engineering Bachelor Sep.1993 ~ Jun. 1997
National Taiwan University Taiwan Chemical Engineering PhD Sep.1997 ~ Oct. 2001
 
Institute Title Dept Year
International Society of Electrochemistry Chairman Division I 2015
National Taiwan University Professor Biomechatronics Engineering Aug 2013 ~ 
National Taiwan University associate professor Bio-Indusrtial Mechatronics Engineering Aug 2009 ~ Jul 2013
Hokkaido University (Japan) Off-campus researcher Chemical Catalysis Research Center Apr 2009 ~ Mar 2010
National Taiwan University Team Leader Bioenergy Research Center Apr 2006 ~ Jul 2011
National Taiwan University Assistant Professor Bio-Indusrtial Mechatronics Engineering Aug 2005 ~ Jul 2009
Academia Sinica postdoc Institute of Biomedical Sciences Dec 2002 ~ Jul 2005
National Cheng- Kung University Postdoc Chemical Engineering Apr 2002 ~ Sep 2002
National Taiwan University Postdoc Chemical Engineering Oct 2001 ~ Mar 2002
Year Award Name Awarding Unit
2013 Excellent Young Scholars Program Award from MOST MOST
2012 Taiwanese Application Scientist France-Taiwan Frontiers of Science
2011 Wu Dazhao Prize from MOST MOST
2011 Excellent Young Scholars Program Award from MOST MOST